Thermo Fisher Scientific Bax Recombinant Mouse Monoclonal Antibody (PSH0-20)
Recombinant mouse monoclonal antibody targeting BAX protein, validated for WB and IHC applications. Expressed in HEK293 cells, purified via Protein A, and supplied as unconjugated liquid form. Ideal for apoptosis-related research with high specificity ...
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | PSH0-20 |
| Immunogen | Synthetic peptide aa 1–50/192 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4, with 0.1% BSA, 40% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_3092988 |
Product Specific Information
- Positive control: Human kidney tissue lysate, Daudi cell lysate, Raji cell lysate, HepG2 cell lysate, human liver carcinoma tissue
- Predicted band size: 21 kDa
- Subcellular Location: Mitochondrion outer membrane, Cytoplasm
Target Information
BAX is a member of the Bcl-2 family and plays an important role in the regulation of apoptosis. Whereas Bcl-2 is considered anti-apoptotic, BAX has a pro-apoptotic function. Apoptosis regulation involves both homo- and heterodimerization of BAX and Bcl-2 isoforms. The Bax gene encodes isoforms Bax alpha (21 kDa) and Bax beta (24 kDa), both containing BH1, BH2, and BH3 domains. Bax beta has a unique carboxyl terminus lacking a hydrophobic transmembrane domain. Overexpression of BAX promotes cell death through dimerization with BCL-2 family proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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