Thermo Fisher Scientific CD137/4-1BB/TNFRSF9 Recombinant Rabbit Monoclonal Antibody (4-1BB, 4552R)
CD137/4-1BB/TNFRSF9 단백질을 인식하는 Thermo Fisher의 재조합 토끼 단클론 항체. Western blot, IHC, ICC, Flow cytometry에 적합. 인간 시료 반응성. Protein A/G 정제, PBS 완충액에 보관. 연구용 전용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–4 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 4-1BB, 4552R |
| Immunogen | Recombinant fragment (aa 19–188) of human CD137 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For immunohistochemistry (PFA-fixed): incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating in 10 mM Tris Buffer with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
CD137 (also known as TNFRSF9 or 4-1BB) is an inducible costimulatory molecule expressed mainly on activated T cells. Its ligand, 4-1BBL, is expressed on activated macrophages, mature B cells, hematopoietic stem cells, and myeloid progenitor cells.
CD137 signaling supports survival and clonal expansion of activated and memory T cells, while modulating myelopoiesis and dendritic cell development. Triggered CD137 induces cytokine release affecting peripheral monocyte survival. Soluble CD137 may act as a negative regulator of immune responses.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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