Thermo Fisher Scientific CD137/4-1BB/TNFRSF9 Recombinant Rabbit Monoclonal Antibody (4-1BB, 4552R)
Recombinant rabbit monoclonal antibody targeting human CD137/4-1BB/TNFRSF9. Validated for WB, IHC, ICC/IF, and Flow Cytometry. High purity (Protein A/G), unconjugated form, suitable for T-cell activation studies. For research use only.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–4 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10^6 cells |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 4-1BB, 4552R |
| Immunogen | A recombinant fragment (around aa 19–188) of human CD137 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Antibody is tested in direct ELISA.
- For Immunohistochemistry (PFA fixed): incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris Buffer with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
CD137 (TNFRSF9, 4-1BB) is an inducible costimulatory molecule expressed mainly on activated T cells. Its ligand, 4-1BBL, is present on activated macrophages, mature B cells, hematopoietic stem cells, and myeloid progenitor cells. CD137 signaling supports the survival and expansion of activated and memory T cells, while modulating myelopoiesis and dendritic cell development. Activation of CD137 induces cytokine release that regulates peripheral monocyte survival. Soluble forms of CD137 may act as negative regulators in immune responses.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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