Thermo Fisher Scientific Vimentin Polyclonal Antibody
Thermo Fisher Scientific의 Vimentin Polyclonal Antibody는 인간을 포함한 다양한 종에 반응하는 고품질 항체로, WB, IHC, ICC/IF, ELISA 등 다양한 응용에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, 안정적인 액상 형태로 장기 보관이 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
| ELISA | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Vimentin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.95 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2792480 |
Product Specific Information
- Keep as concentrated solution.
- Predicted reactivity: Mouse (98%), Rat (98%), Zebrafish (80%), Xenopus laevis (85%), Dog (97%), Chicken (94%), Chimpanzee (100%), Bovine (98%), Guinea pig (96%).
- Positive Control: NT2D1, 293T, HeLa, HepG2, Neuro2A, NIH-3T3, Rat2, C8D30, C2C12.
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is believed to be involved with intracellular transport of proteins between the nucleus and plasma membrane. Unlike other IFP proteins, vimentin is expressed, along with desmin, during early stages of cellular development. During development, vimentin is replaced by tissue-specific IFPs. Vimentin may play a role in steroid synthesis by serving as a storage network for cholesterol-containing lipid droplets. Phosphorylation of vimentin leads to intermediate filament breakdown and cytoskeletal changes facilitating lipid droplet interaction with mitochondria, enhancing steroid synthesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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