Thermo Fisher Scientific Metallothionein Monoclonal Antibody (8D8), PE
Pseudomonas aeruginosa Metallothionein 단백질을 검출하는 PE 결합 단클론 항체. Western blot 및 ELISA에 사용 가능. 높은 특이성과 민감도를 제공하며, 36 kDa 밴드를 인식. 연구용으로만 사용.
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Thermo Fisher Scientific Metallothionein Monoclonal Antibody (8D8), PE
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| ELISA | 1:800 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bacteria |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 8D8 |
| Immunogen | Recombinant full length Pseudomonas aeruginosa Metallothionein protein |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932359 |
Additional Formats
- APC (Cat. MA5-45902)
- FITC (Cat. MA5-45903)
- PerCP (Cat. MA5-45904)
- Custom conjugation available upon request
Product Specific Information
A 1:1,000 dilution of MA5-45905 was sufficient for detection of Metallothionein in 1 µg of purified Pseudomonas aeruginosa Metallothionein (PmtA)-GST tagged protein by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody. Detects a 36 kDa band corresponding to Pseudomonas aeruginosa Metallothionein.
Target Information
Metallothioneins (MTs) are a family of low molecular weight, heavy metal-binding proteins characterized by a high cysteine content and lack of aromatic amino acids. MTs bind 7 to 12 heavy metal atoms per molecule of protein. They are ubiquitous in the animal and plant kingdoms and are also found in prokaryotes. In mammals, the cysteine residues are absolutely conserved and serve to coordinate heavy metal atoms such as zinc, cadmium, and copper via mercaptide linkages.
Usage Notice
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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