Thermo Fisher Scientific ATF2 Monoclonal Antibody (ZY173)
ATF2 단백질을 인식하는 Thermo Fisher Scientific의 단일클론 항체로, 인간 시료에 반응하며 WB 및 IHC(P) 분석에 적합합니다. Jurkat 세포를 양성 대조군으로 사용하며, 핵 내 ATF2 단백질 검출에 유용합니다. 연구용으로만 사용됩니다.
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Applications
Western Blot (WB)
- Tested Dilution: 0.1–1 µg/mL
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 2–10 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | ZY173 |
| Immunogen | Recombinant full-length human ATF2 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with proprietary stabilizer |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C (short term); -20°C (long term), avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Positive control: Jurkat cells
- Cellular location: Nucleus
Target Information
ATF-2 (Activating Transcription Factor 2, also known as CRE-BP1) is a transcription factor that binds to AP-1 and CRE DNA response elements and belongs to the ATF/CREB family of leucine zipper proteins.
ATF-2 interacts with various viral oncoproteins and cellular tumor suppressors and is a target of SAPK/JNK and p38 MAP kinase signaling pathways.
Cellular stress, including genotoxic agents, inflammatory cytokines, and UV irradiation, stimulates ATF-2 transcriptional activity through phosphorylation at Thr69 and Thr71.
Both SAPK and p38 MAPK phosphorylate ATF-2 at these sites in vitro and in transfected cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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