Norgen Hepatitis B virus (HBV) TaqMan PCR Detection Kits

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HBV 감염 검출을 위한 TaqMan PCR 기반 키트로 높은 정확도 제공. 사용이 간편한 Ready-to-use 포맷으로 양성·음성 컨트롤 포함. 연구용으로 PCR 억제 감시 및 품질 검증 가능. -20°C 보관으로 안정적 성능 유지.

카탈로그번호: TM292xx (2개 옵션)
브랜드: Norgen
카테고리: PCR Kits
판매단위: pk

#Norgen #PCR Reagents #Electrophoresis

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Norgen · Norgen Hepatitis B virus (HBV) TaqMan PCR Detection Kits

Norgen Hepatitis B Virus (HBV) TaqMan PCR Detection Kits

SKU: TM29250

Features and Benefits

Detection kits for Hepatitis B virus (HBV)
Available in TaqMan format for quantitative analysis
Ready-to-use format including Master Mix and PCR controls
Specific Primer and Probe mix for the pathogen of interest
Positive and negative controls included for assay validation

Product Description

The Hepatitis B virus (HBV) is mainly transmitted via blood or blood products. Sexual, oral, and perinatal infections are also possible. Early symptoms include appetite loss, vomiting, and abdominal discomfort; about 10–20% of patients develop fever and joint or muscle pain. Jaundice may appear within 2–14 days, occasionally accompanied by itching. Fulminant hepatitis occurs in about 1% of cases and can be fatal. Approximately 5–10% of infected individuals develop chronic liver inflammation, which may progress to cirrhosis or liver carcinoma. HBV is a hepadnavirus with a circular, partially double-stranded DNA genome.

Kit Options

HBV TaqMan PCR Kit (100 reactions)

Ready-to-use format including Master Mix and PCR control
Specific Primer and Probe mix for HBV detection
Positive and negative controls for reagent integrity confirmation

HBV TaqMan PCR Probe/Primer Set and Controls (100 reactions)

Specific Primer/Probe mix and Positive Control for HBV
Includes nuclease-free water
Compatible with Norgen’s PCR Master Mix (#28007) or user-supplied mix

For research use only. Not intended for in vitro diagnostics.

Supporting Data

Figure 1. HBV TaqMan PCR Positive Result
Both PCR signals above the baseline from FAM and HEX channels indicate successful amplification.

상단 그래프: Amplification (FAM channel), Y축 RFU, X축 Cycles
하단 그래프: Amplification (HEX channel), Y축 RFU, X축 Cycles

Figure 2. HBV TaqMan PCR Negative Result
No target DNA detected in FAM channel; amplification signal from HEX indicates successful PCR.

상단 그래프: Amplification (FAM channel), Y축 RFU, X축 Cycles
하단 그래프: Amplification (HEX channel), Y축 RFU, X축 Cycles

Figure 3. HBV TaqMan PCR Inhibition Result
No signal detected in both FAM and HEX channels. Repeat sample preparation using recommended DNA purification kit.

상단 그래프: Amplification (FAM channel), Y축 RFU, X축 Cycles
하단 그래프: Amplification (HEX channel), Y축 RFU, X축 Cycles

Storage Conditions and Product Stability

All components stable for 2 years after production
Store at -20°C upon arrival
Avoid repeated freeze-thaw cycles (>2×) for Master Mix and Positive Control
For intermittent use, aliquot reagents before freezing

Kit Components

Component	Cat. TM29250 (100 rxns)	Cat. TM29210 (100 rxns)
MDx TaqMan 2X PCR Master Mix	2 × 700 μL	–
HBV Primer & Probe Mix	280 μL	280 μL
HBV Positive Control	150 μL	150 μL
Nuclease-Free Water (Negative Control)	1.25 mL	1.25 mL
Product Insert	1	1

Documentation

Protocols

Safety Data Sheets

제품 이미지

Figure 1. HBV TaqMan PCR Kit
Figure 2. HBV TaqMan PCR Kit
Figure 3. HBV TaqMan PCR Kit
HBV TaqMan PCR Kit (100 reactions)
Plasma/Serum Cell-Free Circulating DNA Purification Micro Kit