Thermo Fisher Scientific NSE Polyclonal Antibody
Thermo Fisher Scientific의 NSE Polyclonal Antibody는 인간, 마우스, 랫트 반응성을 가진 rabbit IgG 항체로, WB, ICC/IF, ELISA, IP 등 다양한 응용에 적합합니다. 신경 특이성 에놀라제(NSE)를 표적하며, 신경세포 및 신경내분비세포 연구에 유용합니다. 연구용 전용 제품입니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5 µg–4 µg antibody for 400 µg–600 µg extracts |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 195–434 of human ENO2 (NP_0019661) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 4.27 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.05% ProClin 300 |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2804947 |
Product Specific Information
Immunogen sequence:
VIKDKYGKDA TNVGDEGGFA PNILENSEAL ELVKEAIDKA GYTEKIVIGM DVAASEFYRD GKYDLDFKSP TDPSRYITGD QLGALYQDFV RDYPVVSIED PFDQDDWAAW SKFTANVGIQ IVGDDLTVTN PKRIERAVEE KACNCLLLKV NQIGSVTEAI QACKLAQENG WGVMVSHRSG ETEDTFIADL VVGLCTGQIK TGAPCRSERL AKYNQLMRIE EELGDEARFA GHNFRNPSVLPositive Samples:
HeLa, LO2, SH-SY5Y, U-251MG, Mouse skeletal muscle, Mouse liver, Mouse brain, Rat brain, Rat skeletal muscleCellular Location:
Cell membrane, Cytoplasm
Target Information
Neuron specific enolase (NSE, also known as ENO1, ENO2, ENO3 or enolase alpha, beta, gamma) is an enzyme involved in the glycolytic pathway, catalyzing the conversion of 2-phosphoglycerate to phosphoenolpyruvate. NSE is highly stable in biological fluids and diffuses easily into extracellular and cerebrospinal fluid (CSF) upon neuronal injury.
NSE is expressed primarily in neurons and neuroendocrine cells, and co-expressed with chromogranin A in neuroendocrine neoplasms. Antibodies to NSE are valuable for identifying neuronal cell bodies, developing neuronal lineage, and neuroendocrine cells.
Clinically, NSE serves as a biomarker for neuronal damage and is elevated in conditions such as stroke, hypoxic brain injury, Creutzfeldt-Jakob disease, and certain cancers including neuroblastoma and small cell lung cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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