Thermo Fisher Scientific mTOR Polyclonal Antibody
mTOR 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody로, WB, IHC, ICC/IF, IP 등 다양한 응용 가능. Human, Mouse, Rat 반응성 보유. 항원 친화 크로마토그래피로 정제된 액상 형태이며, 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | 13 publications |
| Immunohistochemistry (IHC) | – | 4 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | – |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | 1 publication |
| Immunoprecipitation (IP) | 1:100–1:500 | 1 publication |
| ChIP assay (ChIP) | Assay-dependent | 1 publication |
| Miscellaneous PubMed (Misc) | – | 2 publications |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 2386–2549 of mTOR (Uniprot ID# P42345) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.05 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2552015 |
Product Specific Information
- Recommended positive controls: 293T, A431, HeLa, HepG2, mouse brain, Flag-human mTOR transfected 293T
- Predicted reactivity: Mouse (99%), Rat (100%), Zebrafish (90%), Sheep (98%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
FRAP1 (mTOR) is a serine/threonine kinase that plays a critical role in cellular growth and proliferation.
Perturbations in the mTOR/PI3-kinase/AKT pathway are associated with numerous forms of cancer.
FRAP1 is also the target of rapamycin and its analogues, which are currently used as immunosuppressants and cancer therapeutics.
Mutations affecting the gene result in Smith-Kingsmore syndrome.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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