
Thermo Fisher Scientific gamma Actin Polyclonal Antibody
Thermo Fisher Scientific의 gamma Actin Polyclonal Antibody는 인간, 생쥐, 랫트에 반응하는 다클론 항체로, Western blot 및 IHC 분석에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, 액상 형태로 제공되어 안정적인 실험 결과를 제공합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:100–1:1,000 |
| Immunohistochemistry (Frozen) (IHC-F) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein fragment corresponding to amino acids 1–375 of gamma Actin (UniProt ID: P63261-1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 10% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2552019 |
Product Specific Information
- Recommended positive controls: A431, NIH-3T3, JC, BCL-1, PC-12, Rat2
- Predicted reactivity: Mouse (100%), Rat (100%), Xenopus laevis (100%), Dog (96%), Chicken (100%), Chimpanzee (100%), Bovine (100%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Actins are highly conserved proteins involved in cell motility and cytoskeletal maintenance.
There are three main actin isoform groups in vertebrates: alpha, beta, and gamma.
- Alpha actins: Found in muscle tissues; major component of the contractile apparatus.
- Beta and gamma actins: Present in most cell types; mediate internal cell motility and cytoskeleton structure.
Actin gamma 1, encoded by this gene, is a cytoplasmic actin found in all cell types.
Mutations are linked to DFNA20/26 (autosomal dominant non-syndromic progressive hearing loss) and Baraitser-Winter syndrome.
Alternative splicing results in multiple transcript variants.
Safety Information
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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