Thermo Fisher Scientific Calnexin Polyclonal Antibody
Calnexin 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. Western blot, IHC, ICC 등 다양한 응용에 적합. 인간, 생쥐, 랫트 반응성. 항원 친화 크로마토그래피로 정제된 액상 형태로 안정적 보관 가능.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:10,000 | View 6 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | View 1 publication |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 243–418 of Calnexin (Uniprot ID: P27824) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.16 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol, 1% BSA |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2552017 |
Product Specific Information
- Recommended positive controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji, NIH-3T3, PC-12, Rat2
- Predicted reactivity: Mouse (93%), Rat (93%), Zebrafish (82%), Xenopus laevis (89%), Dog (94%), Pig (94%), Chicken (91%), Bovine (94%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Calnexin (also known as IP90, p88, p90) is an approximately 90 kDa integral membrane protein of the endoplasmic reticulum (ER). It acts as a molecular chaperone involved in the proper folding and assembly of polypeptides. Calnexin associates with MHC class I heavy chains and partial complexes of T cell receptors and B cell membrane immunoglobulins, but not with completed receptor complexes. It functions to retain incompletely or improperly folded proteins within the ER.
Unlike soluble ER resident proteins containing the KDEL sequence, integral membrane proteins like calnexin lack this motif but possess positively charged cytosolic residues ensuring ER retention. Calnexin consists of a large ER luminal domain (461 amino acids), a transmembrane segment (22 amino acids), and a cytoplasmic tail (89 amino acids).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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