Thermo Fisher Scientific CD326 (EpCAM) Monoclonal Antibody (G8.8), PE-Cyanine5, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant Violet 711
• Brilliant Violet 786
• eFluor 450
• FITC
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Request custom conjugation

Product Specific Information

Description:
The G8.8 monoclonal antibody recognizes the 40 kDa mouse EpCAM (epithelial cellular adhesion molecule), also known as EGP40, 17-1A antigen, TACSTD1, and CD326. The immunogen used was the TE-71 thymic epithelial cell line. CD326 is expressed on most epithelial cells and is considered a pan-carcinoma antigen. It mediates calcium-independent, homophilic cell-cell adhesion and may function as a growth factor receptor. The antigen is being investigated as a target for immunotherapy of human carcinomas. CD326 binds LAIR-1 (CD305) and LAIR-2 (CD306) to inhibit cellular activation and inflammation, playing an important role in tumor biology.

Applications Reported:
Reported for use in flow cytometric analysis.

Applications Tested:
Tested by flow cytometric analysis of the TE-71 cell line. Recommended use ≤0.125 µg/test (100 µL final volume). Optimal concentration should be titrated empirically for each assay (10⁵–10⁸ cells/test).

Light Sensitivity:
This tandem dye is sensitive to photo-induced oxidation. Protect vial and stained samples from light.

Fixation:
Samples can be stored in IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-57) for up to 3 days at 4°C in the dark with minimal impact on brightness and FRET efficiency. Clone-specific performance should be determined empirically.

Excitation / Emission / Laser:
488–561 nm excitation; 667 nm emission; compatible with Blue, Green, and Yellow-Green lasers.

Target Information

Ep-CAM (epithelial adhesion molecule, ESA) is a 40 kDa transmembrane glycoprotein expressed in epithelial tissues. It functions as a calcium-independent homotypic adhesion molecule and influences cell cycle, proliferation, and metabolism by inducing proto-oncogene c-myc and cyclins A/E. Ep-CAM overexpression correlates with enhanced epithelial proliferation and is highly expressed in human carcinomas, serving as a marker for epithelial-lineage tumors. It localizes to the basolateral surface of most simple epithelia and distinguishes adenocarcinomas from pleural mesotheliomas.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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