Thermo Fisher Scientific CD226 (DNAM-1) Monoclonal Antibody (10E5), APC, eBioscience

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.25 µg/test

View 4 publications 4 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

10E5

Conjugate

APC APC APC

View additional formats

• Functional Grade
• Request custom conjugation

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11151707

Product Specific Information

Description: This 10E5 monoclonal antibody reacts with mouse CD226, which is also known as DNAX accessory molecule-1 (DNAM-1). CD226 is an adhesion molecule expressed primarily on T cells, including thymocytes. The highest levels of CD226 are found on naive CD8 T cells. Moreover, expression of CD226 is regulated differentially in CD4 T cells. Under certain culture conditions, CD226 can be upregulated in Th1 and downregulated in Th2 and Th0 cells. This cell surface receptor can also be detected on natural killer cells, some B cell subsets, monocytes, and platelets. Interaction with its ligands, the poliovirus receptor (CD155) and CD112, results in adhesion and cell signaling events that promote innate and adaptive immunity, including differentiation, survival, and cytotoxicity.

The 10E5 monoclonal antibody has been reported to inhibit antigen-specific T cell proliferation and IFN-gamma production in vivo.

Applications Reported: This 10E5 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 10E5 antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

DNAM 1 is a receptor expressed by peripheral blood T lymphocytes that is involved in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T lymphocytes. DNAM 1 is broadly expressed on T cells, NK cells, platelets, monocytes and a subset of B cells. DNAM-1 is also expressed by a subset of CD3 positive thymocytes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.