Thermo Fisher Scientific CD223 (LAG-3) Monoclonal Antibody (eBioC9B7W (C9B7W)), APC, eBioscience

Thermo Fisher Scientific CD223 (LAG-3) Monoclonal Antibody (eBioC9B7W (C9B7W)), APC, eBioscience

Applications and Tested Dilution

• Flow Cytometry (Flow): 0.5 µg/test
• Publications: View 11 publications

Product Specifications

Available Formats

• Unconjugated
• APC-eFluor 780
• Brilliant Violet 480
• eFluor 450
• FITC
• Functional Grade
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Super Bright 702
• Request custom conjugation available

Product Specific Information

The eBioC9B7W monoclonal antibody recognizes mouse CD223 (LAG-3, LAG3) protein expressed by activated α/β-TCR bearing T cells, a subset of γ/δ-TCR bearing T cells, and NK cells.
CD223 is a 70 kDa type I transmembrane protein similar to CD4. In mice, proteolytic cleavage in the D4 domain produces a 54 kDa extracellular fragment and a 16 kDa intracellular fragment. The 54 kDa fragment can remain membrane-associated or be released as soluble CD223.

CD223 binds to MHC class II with higher affinity than CD4, contributing to negative regulation of T-cell activation and homeostatic proliferation. It is also expressed on CD4+CD25+ regulatory T cells and may play a role in their regulatory function.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on stimulated mouse splenocytes at ≤0.5 µg/test (100 µL final volume).
Cell number range: 10⁵–10⁸ cells/test.
Careful titration recommended for optimal performance.

Excitation: 633–647 nm
Emission: 660 nm
Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

LAG-3 is a 70-kDa surface glycoprotein of the Ig superfamily with homology to CD4.
It binds MHC class II with higher affinity than CD4 and negatively regulates T-cell activation and proliferation.
Surface expression is observed on activated T cells (including regulatory T cells) and NK cells.
CD8+ T cells typically express higher LAG-3 levels than CD4+ T cells.
Coexpression of LAG-3 and CD49b identifies Type 1 regulatory T cells (Tr1) in both human and mouse.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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