Thermo Fisher Scientific CD223 (LAG-3) Monoclonal Antibody (eBioC9B7W (C9B7W)), APC, eBioscience
마우스 CD223(LAG-3)을 인식하는 eBioC9B7W 단클론 항체로, Flow Cytometry에 최적화된 APC 형광 표지 제품입니다. 높은 특이성과 안정성을 제공하며, T세포 활성 조절 연구에 적합합니다. 0.5 µg/test로 사용 가능하며, 4°C 보관 권장.
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- 17-2231-8x (2개 옵션)
- 판매단위
- pk
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2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD223 (LAG-3) Monoclonal Antibody (eBioC9B7W (C9B7W)), APC, eBioscience
Thermo Fisher Scientific CD223 (LAG-3) Monoclonal Antibody (eBioC9B7W (C9B7W)), APC, eBioscience
Applications and Tested Dilution
- Flow Cytometry (Flow): 0.5 µg/test
- Publications: View 11 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG1, kappa |
| Recommended Isotype Control | Rat IgG1 kappa Isotype Control (eBRG1), APC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBioC9B7W (C9B7W) |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2573183 |
Available Formats
- Unconjugated
- APC-eFluor 780
- Brilliant Violet 480
- eFluor 450
- FITC
- Functional Grade
- PE
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Super Bright 702
- Request custom conjugation available
Product Specific Information
The eBioC9B7W monoclonal antibody recognizes mouse CD223 (LAG-3, LAG3) protein expressed by activated α/β-TCR bearing T cells, a subset of γ/δ-TCR bearing T cells, and NK cells.
CD223 is a 70 kDa type I transmembrane protein similar to CD4. In mice, proteolytic cleavage in the D4 domain produces a 54 kDa extracellular fragment and a 16 kDa intracellular fragment. The 54 kDa fragment can remain membrane-associated or be released as soluble CD223.
CD223 binds to MHC class II with higher affinity than CD4, contributing to negative regulation of T-cell activation and homeostatic proliferation. It is also expressed on CD4+CD25+ regulatory T cells and may play a role in their regulatory function.
Applications Reported: Flow cytometric analysis
Applications Tested: Tested on stimulated mouse splenocytes at ≤0.5 µg/test (100 µL final volume).
Cell number range: 10⁵–10⁸ cells/test.
Careful titration recommended for optimal performance.
Excitation: 633–647 nm
Emission: 660 nm
Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered
Target Information
LAG-3 is a 70-kDa surface glycoprotein of the Ig superfamily with homology to CD4.
It binds MHC class II with higher affinity than CD4 and negatively regulates T-cell activation and proliferation.
Surface expression is observed on activated T cells (including regulatory T cells) and NK cells.
CD8+ T cells typically express higher LAG-3 levels than CD4+ T cells.
Coexpression of LAG-3 and CD49b identifies Type 1 regulatory T cells (Tr1) in both human and mouse.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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