Thermo Fisher Scientific P4HTM Polyclonal Antibody
P4HTM 단백질을 인식하는 Rabbit Polyclonal 항체로, WB 및 IHC(P) 실험에 적합합니다. 인간, 마우스, 랫트 반응성이 있으며, 고순도 친화 크로마토그래피로 정제되었습니다. 세포 내 산소 감지 및 HIF 경로 연구에 활용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human P4HTM (Accession Q9NXG6), corresponding to amino acid residues P378–G428. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2900344 |
Product Specific Information
Antibody detects endogenous levels of total P4HTM.
Target Information
Prolyl hydroxylase 4 (P4HTM) is an enzyme that modifies HIF-alpha, playing a critical role in oxygen homeostasis. Unlike classic prolyl hydroxylases that modify collagen in the endoplasmic reticulum, P4HTM acts intracellularly on HIF proteins. Under normoxic conditions, prolyl hydroxylases hydroxylate HIF-alpha, leading to its degradation via the von Hippel-Lindau ubiquitylation complex. Under hypoxic conditions, this hydroxylation is reduced, stabilizing HIF and promoting angiogenesis. P4HTM may therefore function as a cellular oxygen sensor involved in regulating HIF stability.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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