Thermo Fisher Scientific Phosphotyrosine Monoclonal Antibody (IG2)
다양한 종과 조직에서 인산화 티로신을 검출하는 Mouse IgG1 단클론 항체. ELISA, Western blot, ICC/IF, IP 등 다중 응용 가능. 고순도 친화 크로마토그래피 정제 및 4°C 보관. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:100–1:1,000 | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 | - |
| Flow Cytometry (Flow) | Assay-dependent | - |
| ELISA | 1 µg/mL | - |
| Immunoprecipitation (IP) | 2 µg | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Chemical |
| Published Species | Chemical |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | IG2 |
| Immunogen | O-phospho-L-tyrosine and O-phospho-DL-tyramine |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 40% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
| RRID | AB_326368 |
Product Specific Information
MA4-003 detects phosphotyrosine from most species and tissues.
It has been successfully used in ELISA, Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, and FACS procedures.
This antibody detects proteins containing phosphorylated tyrosine residues.
The MA4-003 antigen is O-phospho-L-tyrosine and O-phospho-DL-tyramine.
Target Information
Tyrosine phosphorylation plays a critical role in signal transduction from transmembrane receptors and in oncogene tyrosine kinase-mediated transformation.
Phosphorylation of specific tyrosine residues is a key mechanism in mitogenesis, cell cycle progression, and oncogenic transformation.
Antibodies recognizing phosphorylated tyrosine residues are essential tools for studying tyrosine-phosphorylated proteins and related biochemical pathways.
The fluorescein (FITC) conjugate of clone PY20 anti-phosphotyrosine is particularly useful for immunohistochemical and immunocytochemical detection of P-Tyr proteins when secondary antibody use is undesirable.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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