Thermo Fisher Scientific Phosphotyrosine Monoclonal Antibody (IG2)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:1,000

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Immunocytochemistry (ICC/IF)

1:50 - 1:200

-

Flow Cytometry (Flow)

Assay-dependent

-

ELISA (ELISA)

1 µg/mL

-

Immunoprecipitation (IP)

2 µg

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Product Specifications

Species Reactivity

Chemical

Published species

Chemical

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

IG2

Immunogen

O-phospho-L-tyrosine and O-phospho-DL-tyramine

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 40% glycerol

Contains

0.05% sodium azide

Storage conditions

4° C

Shipping conditions

Wet ice

RRID

AB_326368

Product Specific Information

MA4-003 detects phosphotyrosine from most species and tissues.

MA4-003 has been successfully used in ELISA, Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, and FACS procedures. This antibody detects proteins which contain phosphorylated tyrosine residues.

The MA4-003 antigen is O-phospho-L-tyrosine and O-phospho-DL-tyramine.

Target Information

The role of tyrosine phosphorylation in transduction of the mitogenic signal from transmembrane receptors and in transformation by oncogene tyrosine kinases has been the subject of intense investigation for several years. While the phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine -phosphorylated proteins and the biochemical pathways in which they function. The fluorescein (FITC) conjugate of clone PY20 anti-phosphotyrosine is especially useful for the detection of these P-Tyr proteins in immunohistochemical and immunocytochemical protocols in situations wherein the use of a secondary antibody would complicate detection of the protein(s) of interest.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.