Thermo Fisher Scientific TOPORS Polyclonal Antibody
TOPORS 단백질을 표적하는 Rabbit Polyclonal 항체로, WB, IHC, IP 등 다양한 응용에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, 4°C에서 보관. 인간 단백질과 반응하며, 세포 성장 및 p53 안정성 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution / Amount |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:100–1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunoprecipitation (IP) | 5–15 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 995 and 1045 of Human topoisomerase I binding, arginine/serine-rich |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | TBS, pH 7.0–8.0, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Target Information
TOPORS functions as an E3 ubiquitin-protein ligase and E3 SUMO1-protein ligase. It likely acts as a tumor suppressor involved in cell growth, proliferation, and apoptosis by regulating p53/TP53 stability via ubiquitin-dependent degradation. It may also regulate chromatin modification through sumoylation of chromatin-associated proteins and participate in DNA damage-induced cell death through IKBKE sumoylation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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