Thermo Fisher Scientific SF1 Polyclonal Antibody
SF1 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, IP 등 다양한 응용에 사용 가능. Human과 Mouse에 반응하며, 항원 친화 크로마토그래피로 정제됨. 액상 형태로 제공되며 4°C에서 보관. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:100–1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 25 and 75 of Human Splicing Factor 1 (UniProt ID: Q15637-1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | TBS, pH 7.0–8.0, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections.
Target Information
Splicing factor 1 (SF1) is a nuclear pre-mRNA splicing factor that specifically recognizes the intron branch point sequence at the 3′ splice site, in cooperation with the large subunit of U2 auxiliary factor (U2AF). It is essential for the early stages of spliceosome assembly and contributes to nuclear pre-mRNA retention and transcriptional repression.
The protein contains:
- An N-terminal U2AF ligand motif
- A central hnRNP K homology motif and quaking 2 region that bind the branch-site adenosine
- A zinc knuckle domain
- A C-terminal proline-rich domain
Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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