Thermo Fisher Scientific RhoA Monoclonal Antibody (1B8-1C7)

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:2,000
• View 5 publications

Immunoprecipitation (IP)

• Tested Dilution: —
• View 1 publication

Product Specifications

Product Specific Information

• By Western blot, MA1-134 detects recombinant RhoA, but not recombinant RhoB or RhoC.
• In mouse and rat kidney tissue lysates, detects a ~22 kDa band corresponding to RhoA.
• A nonspecific band at ~55 kDa may also appear in kidney lysates.

Target Information

RhoA regulates signal transduction pathways linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. It plays a crucial role in cytokinesis by controlling myosin contractile ring formation and cleavage furrow development. RhoA is required for apical junction formation in keratinocyte adhesion and serves as a target for Yersinia pestis and Yersinia pseudotuberculosis effectors. It activates PKN2 kinase and may regulate PLCE1 activity. Activated by ARHGEF2, RhoA is essential for SPATA13-mediated cell migration and adhesion dynamics. The MEMO1–RHOA–DIAPH1 pathway supports ERBB2-dependent microtubule stabilization at the cell cortex, controlling APC and CLASP2 localization for microtubule capture and stabilization.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.