Thermo Fisher Scientific RhoA Monoclonal Antibody (1B8-1C7)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 5 publications 5 publications

Immunoprecipitation (IP)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Dog, Human, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

1B8-1C7

Immunogen

Full-length recombinant His-tagged human RhoA if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:RhoA,uniProtId:P61586-1,ncbiNodeId:9606,antigenRange:1-193,antigenLength:193,antigenImageFileName:MA1-134_RhoA_P61586-1_House_mouse.svg,antigenImageFileNamePDP:MA1-134_RhoA_P61586-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2536840

Product Specific Information

By Western blot, MA1-134 detects recombinant RhoA, but not recombinant RhoB or recombinant RhoC.

By Western blot on mouse and rat kidney whole tissue lysates, MA1-134 detects a band of ~22kD corresponding to the expected molecular weight of RhoA. A nonspecific band at ~55kD was also detected in both kidney lysates.

Target Information

RhoA regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. It is involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis, and plays an essential role in cleavage furrow formation. RhoA is required for the apical junction formation of keratinocyte cell-cell adhesion, and serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. RhoA stimulates PKN2 kinase activity and may be an activator of PLCE1. It is activated by ARHGEF2, which promotes the exchange of GDP for GTP. It is essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.