Thermo Fisher Scientific SCUBE3 Polyclonal Antibody

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:50

-

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human SCUBE3. Recombinant protein control fragment (Product #RP-94883). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:SCUBE3,uniProtId:Q8IX30-1,ncbiNodeId:9606,antigenRange:476-566,antigenLength:993,antigenImageFileName:PA5-59221_SCUBE3_Q8IX30-1_Rabbit.svg,antigenImageFileNamePDP:PA5-59221_SCUBE3_Q8IX30-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2647040

Product Specific Information

Immunogen sequence: VLSIKQRASF KIKDAKCRLH LRNKGKTEEA GRITGPGGAP CSECQVTFIH LKCDSSRKGK GRRARTPPGK EVTRLTLELE AEVRAEETTA S

Highest antigen sequence identity to the following orthologs: Mouse - 89%, Rat - 90%.

Target Information

SCUBE3 is a member of a family of secreted glycoproteins that contain N-terminal EGF-like repeats and C-terminal cysteine-rich motifs and CUB domain and is highly expressed in primary osteoblasts and bones, and to a lesser extent in heart. Other studies have shown that overexpression of SCUBE3 in mice induced cardiac hypertrophy, suggesting that it may also play a role in the regulation of cardiac growth. SCUBE3 has been shown to be an endogenous TGF-Beta receptor ligand and is thought to promote lung cancer cell mobility and invasiveness. In lung cancer cells, the secreted SCUBE3 protein was cleaved by MMP2 and MMP9, allowing the activation of the TGF-Beta receptor, the increase of Smad2/3 transcriptional activity and the upregulation of expression of proteins such as TGF-Beta1, VEGF, Snail, and Slug.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.