Thermo Fisher Scientific Phospho-CD3z (Tyr142) Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

A suggested positive control for Western blot is JK or K562 cells.

Target Information

The CD3z complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits (CD3 gamma, CD3 delta, CD3 epsilon, and CD3 zeta). These subunits are members of the immunoglobulin superfamily encoded by closely linked genes on human chromosome 11.

The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules, mediated in part by a double tyrosine-based motif. CD3 may play a role in TCR-induced growth arrest, cell survival, and proliferation. The CD3 antigen is present on 68–82% of normal peripheral blood lymphocytes, 65–85% of thymocytes, and Purkinje cells in the cerebellum, but not on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. Diseases associated with CD3z include Immunodeficiency 25 and T-B+ Severe Combined Immunodeficiency due to CD3delta/CD3epsilon/CD3zeta.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.