Thermo Fisher Scientific Osteopontin Recombinant Rabbit Monoclonal Antibody (001)
인체 Osteopontin 단백질을 인식하는 재조합 토끼 단일클론 항체로 높은 특이성과 감도를 제공. IHC, Flow Cytometry, ELISA에 적합하며 보존제가 없는 액상 형태. 장기간 -20°C 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000–1:4,000 |
| Flow Cytometry (Flow) | 1:25–1:100 |
| ELISA | 1:5,000–1:10,000 |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 001 |
| Immunogen | Recombinant Human Osteopontin/SPP1/ETA-1 protein (Met1–Asn314) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term; for long term storage, store at -20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2785421 |
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%–0.1%).
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned and screened to select the best candidates for production.
Advantages of recombinant rabbit monoclonal antibodies:
- Better specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to larger rabbit immune repertoire
This antibody has specificity for Human Osteopontin/SPP1/ETA-1.
Target Information
Osteopontin is an arginine-glycine-aspartic acid (RGD)-containing glycoprotein that interacts with integrins and CD44 as major receptors. It plays a role in bone mineralization, cell adhesion, migration, chronic inflammation, and cancer transformation. Proteolytic cleavage by thrombin and matrix metalloproteinases near the RGD sequence modulates its integrin-binding properties. Thrombin-cleaved fragments are overexpressed in malignant glial tumors, providing survival advantages and novel substrates for plasmin and cathepsin D.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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