Thermo Fisher Scientific IL-8 (CXCL8) Monoclonal Antibody (344609)

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 1 publication 1 publication

Neutralization (Neu)

0.08-0.32 µg/mL

-

Product Specifications

Species Reactivity

Cat

Published species

Rat

Host/Isotype

Rat / IgG2b

Class

Monoclonal

Type

Antibody

Clone

344609

Immunogen

E. coli-derived recombinant feline CXCL8/IL-8 Ala23-Ala101 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:IL-8,uniProtId:Q9XSX5-1,ncbiNodeId:9685,antigenRange:23-101,antigenLength:101,antigenImageFileName:MA5-24081_IL-8_Q9XSX5-1_Rat.svg,antigenImageFileNamePDP:MA5-24081_IL-8_Q9XSX5-1_Rat_PDP.jpeg,sortOrder:3}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.5 mg/mL

Purification

Protein A/G

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2576461

Product Specific Information

In direct ELISAs and Western blots, no cross-reactivity with over 75 tested chemokines of human, mouse, rat, porcine, or viral origin was observed.

Reconstitute at 0.5 mg/mL in sterile PBS.

Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.

Target Information

Interleukin 8 (IL-8, CXCL8) is a 72 amino acid pro-inflammatory factor which belongs to the CXC subfamily of chemokines, and are bound by the cell surface receptors IL-8RA and IL-8RB. IL-8 functions as a chemoattractant and potent angiogenic factor. The expression and secretion of IL-8 can be induced by diverse inflammatory stimuli in many cells, including macrophages and endothelial cells. In endothelial cells, IL-8 is present in storage vesicles called Weibel-Palade bodies. IL-8, first isolated from osteosarcoma cells, contains the ELR-motif (N-terminal Glu-Leu-Arg amino acid sequence) and signals through the CXCR1 and CXCR2 receptors. Previous nomenclature for IL-8 includes neutrophil activating protein 1 (NAP-1), granulocyte chemotactic protein 1 (GCP-1), monocyte-derived neutrophil-activating peptide (MONAP) and protein 3-10C. IL-8 and ten other members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q. Cancer studies have demonstrated a role for IL-8 in the angiogenesis and growth of tumours, and IL-8 is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.