PolarScreen™ PPARγ-Competitor Assay Kit, Green

The PPARγ (Peroxisome Proliferator-Activated Receptor-gamma) Competitor Assay, Green uses the human-derived recombinant PPARγ ligand-binding domain (PPARγ-LBD) tagged with an N-terminal자세히 알아보기

The PPARγ (Peroxisome Proliferator-Activated Receptor-gamma) Competitor Assay, Green uses the human-derived recombinant PPARγ ligand-binding domain (PPARγ-LBD) tagged with an N-terminal GST-tag and a novel, tight-binding, selective fluorescent PPARγ ligand (Fluormone™ PPARγ Green) in a homogenous, mix-and-read assay format. PPARγ-LBD/Fluormone™ PPARγ Green complex produces high polarization. This complex is then added to individual test compounds in microwell plates. Competitors displace the fluorescent Fluormone™ PPARγ Green Ligand from the PPARγ-LBD/Fluormone™ PPARγ Green complex, causing the fluorescent ligand to tumble rapidly during its fluorescence lifetime, resulting in a low polarization value. Noncompetitors will not displace the fluorescent ligand from the complex, so the polarization value remains high. The shift in polarization value in the presence of the test compounds is used to determine relative affinity of test compounds for the PPARγ-LBD.

사양

색상Green

검출 방법Fluorescence

용도(장비)Microplate Reader

수량800 x 20 μL

포장384-well Plate

어세이 입력Biochemical competitive binding

여기/방출485/535

유전자 ID(Entrez)5468

판독End Point

배송 조건Dry Ice

타겟 입력PPARG, PPAR gamma, NR1C3

제품라인PolarScreen™

용도(애플리케이션)Competitive Binding Assay, Fluorescence Polarization

리간드PPAR

제품 유형Assay Kit

Unit SizeEach