Thermo Fisher Scientific MIA Polyclonal Antibody

Applications

• Western Blot (WB): Tested dilution 1:500–1:10,000

Product Specifications

Product Specific Information

• Recommended positive controls: A375, human MIA-transfected 293T.
• Predicted reactivity: Mouse (91%), Rat (93%), Rhesus Monkey (99%), Bovine (90%).
• Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Tumorigenesis involves a network of membrane, cytosolic, and nuclear-associated factors regulating proliferation and cell-matrix interaction through signaling cascades. Melanoma inhibitory activity (MIA), also known as cartilage-derived retinoic acid-sensitive protein (CD-RAP), is a Src homology 3 (SH3)-like domain-containing protein secreted from chondrocytes and malignant melanoma cells.
MIA is translated as a 131-amino acid pro-form and processed into a mature 107-amino acid protein after cleavage of a secretion signal. It is expressed during chondrogenesis and in mature chondrocytes, indicating its role in maintaining normal cartilage cell phenotype. MIA mRNA is also present in carcinomas of the colon, ovary, kidney, and head/neck, and may serve as a marker to monitor melanomic activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.