Thermo Fisher Scientific CD298 Monoclonal Antibody (2D3B5)
CD298 단백질을 표적으로 하는 마우스 단클론 항체로, Western blot, IHC, Flow cytometry, ELISA에 사용 가능. 고순도 Protein G 정제, 1 mg/mL 농도, PBS buffer에 0.05% sodium azide 포함. 연구용으로 세포막 Na+/K+ ATPase β3 서브유닛 검출에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | |
| Immunohistochemistry (IHC) | 1:200–1:1,000 | |
| Flow Cytometry (Flow) | Assay-dependent | |
| ELISA | 1:10,000 |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2D3B5 |
| Immunogen | Purified recombinant fragment of human CD298 (AA: extra 57–279) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4 °C (short term). For long-term storage, store at −20 °C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_3091002 |
Target Information
The protein encoded by this gene belongs to the family of Na⁺/K⁺ and H⁺/K⁺ ATPase beta chain proteins, specifically the Na⁺/K⁺-ATPase subfamily. Na⁺/K⁺-ATPase is an integral membrane protein responsible for maintaining electrochemical gradients of sodium and potassium ions across the plasma membrane. These gradients are essential for osmoregulation, sodium-coupled transport of various molecules, and electrical excitability of nerve and muscle cells.
This enzyme consists of two subunits: a large catalytic alpha subunit and a smaller glycoprotein beta subunit. The beta subunit regulates the number of sodium pumps transported to the plasma membrane by forming alpha/beta heterodimers. The glycoprotein subunit is encoded by multiple genes, and this particular gene encodes the beta 3 subunit. A pseudogene for this gene is located on chromosome 2.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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