Thermo Fisher Scientific APAF1 Monoclonal Antibody (2H9A1)
APAF1 단백질을 인식하는 Thermo Fisher Scientific의 Mouse Monoclonal Antibody(2H9A1). Western blot, ICC/IF, Flow cytometry, ELISA에 사용 가능. 고순도 Protein G 정제, 1 mg/mL 농도, PBS buffer 및 sodium azide 포함. 세포사멸 연구용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| Flow Cytometry (Flow) | 1:200–1:400 |
| ELISA | 1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2H9A1 |
| Immunogen | Purified recombinant fragment of human APAF1 (AA: 1138–1237) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_3091342 |
Target Information
The APAF1 gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains WD-40 domains, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, APAF1 forms an oligomeric apoptosome that activates caspase 9, triggering the caspase cascade leading to apoptosis. Alternative splicing results in several isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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