Thermo Fisher Scientific Mouse IL-12 p70 (Carrier-Free) Recombinant Protein, eBioscience

Applications

Tested Dilution

Publications

Functional Assay (Functional)

Assay-Dependent

-

Control (Ctrl)

Assay-Dependent

-

Miscellaneous PubMed (Misc)

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View 3 publications 3 publications

Product Specifications

Published species

Not Applicable

Expression System

Insect cells

Class

Recombinant

Type

Protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Storage conditions

-80°C

Shipping conditions

Dry ice

Product Specific Information

Description: Interleukin-12 (IL-12) is a heterodimeric 70 kD (p70) cytokine composed of two covalently linked, glycosylated chains, 40kD (p40) and 35-kD (p35). IL-12 is mainly produced by monocytes, macrophages, and dendritic cells in response to bacterial products such as lipopolysaccharides (LPS), to intracellular pathogens or upon interaction with activated T cells. IL-12 was originally discovered because of its ability to induce interferon-gamma (IFN-g) production, cell proliferation, and cytotoxicity mediated by natural killer cells and T cells. It is now established that IL-12 also plays a key role in the development of Th1 responses, leading to IFN-g and IL-2 production. These cytokines can in turn promote T-cell responses and macrophage activation.

eBioscience`s recombinant mouse IL-12 p70 is produced in baculovirus-infected insect cells as an authentic heterodimer of precursor p35 and p40 subunits using a dual promoter expression system. It is distinct from other available forms of the protein in that it is expressed as a true heterodimer, as opposed to a single-chain, pseudo-heterodimer in which the subunits are joined by an artificial linker.

Applications Reported: Recombinant mouse IL-12 p70 is biologically active.

Applications Tested: The ED50 of this protein, as measured by IFN gamma induction assay in mouse splenocytes, is less than or equal to 175 pg/mL. This corresponds to a specific activity of greater than or equal to 5.7 x 10e6 Units/mg.

Source: Insect cells infected with baculovirus: mouse p40, amino acids met 1-Ser 335, accession # NM_008352 was co-expressed with mouse p35, amino acids met 1-Ala 215, accession # NM_008351.

Bioactivity: The ED50 of this protein, as measured by IFN gamma induction assay in mouse splenocytes, is less than or equal to 175 pg/mL. This corresponds to a specific activity of greater than or equal to 5.7 x 10e6 Units/mg.

Endotoxin: Less than 0.01 ng/ug cytokine as determined by the LAL assay. Purity: >98% as determined by SDS-PAGE.

Molecular Weight: The heterodimeric, p40, amino acids met 23-ser 335, cystine linked to p35, amino acids arg 23-ala 215, has a predicted molecular mass of 57,496. On non-reducing SDS-PAGE the heterodimeric cystine-linked protein migrates as a 65 kDa protein. The DTT reduced protein migrates as 43 kDa and 24 kDa polypeptides.

Storage and handling: For best recovery, quick-spin vial prior to opening. Use in a sterile environment.

Target Information

IL-12 p70, also known as interleukin-12, is a cytokine that plays a critical role in the immune system`s response to infections and cancer. It is produced by immune cells, including dendritic cells, macrophages, and B cells, in response to certain pathogens and tumor cells. IL-12 p70 stimulates the production of interferon-gamma and other cytokines, which promote the activation and proliferation of T cells and natural killer cells. IL-12 p70 has been studied as a potential therapeutic target for various diseases and conditions, including infectious diseases, autoimmune disorders, and cancer. It has been shown to enhance immune responses against certain infections and tumors, and to modulate inflammatory responses in autoimmune diseases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.