Thermo Fisher Scientific Donkey anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody, DyLight 755
DyLight 755 형광 표지를 가진 Donkey anti-Human IgG (H+L) 2차 항체로, Western blot, IHC, ICC, Flow cytometry 등에 사용 가능. 다양한 종에 대해 교차 흡착 처리되어 비특이적 반응 최소화. 높은 순도와 안정적인 형광 신호 제공.
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Thermo Fisher Scientific Donkey anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody, DyLight 755
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:20,000 |
| Immunohistochemistry (IHC) | 1:50–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:500 |
| Flow Cytometry (Flow) | 1:50–1:200 |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Donkey / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Human IgG-heavy and light chain |
| Conjugate | DyLight™ 755 |
| Excitation / Emission Max | 760 / 789 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 6.8–7.4, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2556711 |
| Target | IgG |
| Cross Adsorption | Against bovine, chicken, goat, mouse, rabbit, rat and sheep |
| Antibody Form | Whole Antibody |
Product Specific Information
This antibody is cross-adsorbed and exhibits minimal reactivity to bovine, chicken, goat, mouse, rabbit, rat, and sheep.
Target Information
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins. They are useful in detection, sorting, or purification of the specified target.
Secondary antibodies offer versatility for multiple detection systems (e.g., HRP, AP, fluorescence) and provide enhanced sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
Typically, secondary antibodies are generated by immunizing the host animal with pooled immunoglobulins from the target species and can be further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to produce highly specific reagents.
Additional Formats
- DyLight 488
- DyLight 550
- DyLight 594
- DyLight 650
- DyLight 680
- DyLight 800
- Custom conjugation available upon request
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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배송/결제/교환/반품 안내
배송 정보
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