Thermo Fisher Scientific Goat anti-Human IgG Fc Cross-Adsorbed Secondary Antibody, DyLight 488
DyLight 488 형광으로 표지된 염소 유래 anti-Human IgG Fc 2차 항체로, Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 사용 가능. 여러 종과 교차 반응을 최소화하며, 높은 특이성과 신호 증폭 효율을 제공.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:20,000 |
| Immunohistochemistry (IHC) | 1:50–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:500 |
| Flow Cytometry (Flow) | 1:50–1:200 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Human IgG-Fc Fragment |
| Conjugate | DyLight™ 488 |
| Excitation / Emission Max | 492 / 519 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 6.8–7.4, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2556714 |
| Target | IgG Fc |
| Cross Adsorption | Against bovine, chicken, horse, mouse, rabbit, rat, and sheep |
| Antibody Form | Whole Antibody |
Product Specific Information
This antibody is cross-adsorbed and exhibits minimal reactivity to bovine, chicken, horse, mouse, rabbit, rat, and sheep.
Target Information
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins. They are useful in detection, sorting, or purification of human Ig targets. Secondary antibodies enhance versatility and sensitivity by enabling multiple detection systems (e.g., HRP, AP, fluorescence) and signal amplification. Typically, these antibodies are produced by immunizing the host animal with pooled human immunoglobulins and further purified or modified (e.g., immunoaffinity chromatography, fragmentation, or label conjugation) for high specificity.
Additional Formats
- AP
- Biotin
- DyLight 550
- DyLight 594
- DyLight 650
- DyLight 680
- DyLight 755
- DyLight 800
- FITC
- HRP
- TRITC
- Custom conjugation available upon request
Regulatory Note
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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배송/결제/교환/반품 안내
배송 정보
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