Thermo Fisher Scientific TOP2A Recombinant Rabbit Monoclonal Antibody (SY27-00)
Recombinant rabbit monoclonal antibody for TOP2A detection with high specificity and sensitivity. Suitable for WB and IHC applications. Expressed in HEK293 cells, purified by Protein A, and supplied as a liquid form for research use only.
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Applications
Western Blot (WB)
- Tested Dilution: 1:1,000–1:2,000
- Publications: References
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:50–1:200
- Publications: References
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SY27-00 |
| Immunogen | Synthetic peptide within Human Topoisomerase II alpha (residues 1,482–1,531) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% Sodium Azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2809389 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned and screened to select optimal candidates.
Advantages include:
- High specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to the rabbit immune repertoire
Target Information
Topo II alpha is crucial for DNA synthesis, transcription, and chromosomal segregation during mitosis. It serves as a sensitive and specific marker for late S-, G2-, and M-phases in transformed and normal cells.
Topo II alpha is also associated with tumor cell drug resistance.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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