Thermo Fisher Scientific GPAM Polyclonal Antibody
Thermo Fisher Scientific의 GPAM Polyclonal Antibody는 Western blot과 IHC에 적합한 토끼 유래 다클론 항체입니다. Human, Mouse, Rat 시료에 반응하며, 항원 친화 크로마토그래피로 정제되었습니다. GPAT1 단백질 연구용으로 사용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–2 µg/mL | View 4 publications |
| Immunohistochemistry (IHC) | 2.5 µg/mL | – |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A 15 amino acid peptide near the carboxy terminus of the human GPAT1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | 2–8°C for up to 3 months; for long-term storage, store at –20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11155813 |
Product Specific Information
- Suggested positive control: Rat brain tissue lysate
- PA5-20524 can be used with blocking peptide PEP-0644.
Target Information
Glycerol-3-phosphate acyltransferase 1 (GPAT1), one of four known GPAT isoforms, is located on the mitochondrial outer membrane, allowing reciprocal regulation with carnitine palmitoyltransferase-1. It is thought to be critical for the development of hepatic steatosis; GPAT1 overexpression can lead to hepatic and peripheral insulin resistance. GPAT1 is transcriptionally upregulated by insulin and sterol regulatory element binding protein (SREBP-1) and downregulated by AMP-activated protein kinase. Mice deficient in GPAT1 exhibit decreased triacylglycerol (TAG) in cardiomyocytes even in high-fat diets, suggesting that GPAT1 contributes significantly to TAG accumulation in heart tissue during lipogenic or high-fat diets. At least two isoforms of GPAT1 are known to exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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