Thermo Fisher Scientific SDHB Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 29-280 of human SDHB (NP_0029912) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:SDHB,uniProtId:P21912-1,ncbiNodeId:9606,antigenRange:29-280,antigenLength:280,antigenImageFileName:PA5-88051_SDHB_P21912-1_Rabbit.svg,antigenImageFileNamePDP:PA5-88051_SDHB_P21912-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2.82 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.05% ProClin 300

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2804617

Product Specific Information

Immunogen sequence: AQTAAATAPR IKKFAIYRWD PDKAGDKPHM QTYEVDLNKC GPMVLDALIK IKNEVDSTLT FRRSCREGIC GSCAMNINGG NTLACTRRID TNLNKVSKIY PLPHMYVIKD LVPDLSNFYA QYKSIEPYLK KKDESQEGKQ QYLQSIEERE KLDGLYECIL CACCSTSCPS YWWNGDKYLG PAVLMQAYRW MIDSRDDFTE ERLAKLQDPF SLYRCHTIMN CTRTCPKGLN PGKAIAEIKK MMATYKEKKA SV; Positive Samples: HepG2, HeLa, U-87MG, Mouse brain, Mouse liver, Mouse kidney, Mouse heart, Rat liver, Rat brain; Cellular Location: Matrix side, Mitochondrion inner membrane, Peripheral membrane protein

Target Information

Complex II of the respiratory chain, which is specifically involved in the oxidation of succinate, carries electrons from FADH to CoQ. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. The iron-sulfur subunit is highly conserved and contains three cysteine-rich clusters which may comprise the iron-sulfur centers of the enzyme. Sporadic and familial mutations in this gene result in paragangliomas and pheochromocytoma, and support a link between mitochondrial dysfunction and tumorigenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.