Thermo Fisher Scientific Pyruvate Carboxylase Polyclonal Antibody
Pyruvate Carboxylase 단백질을 검출하는 Rabbit Polyclonal Antibody로 WB, IHC, ICC, IP 등 다양한 응용에 사용 가능. 인간 및 마우스 반응성. 항원 친화 크로마토그래피로 정제되었으며, -20°C에서 보관. 연구용으로만 사용.
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Thermo Fisher Scientific Pyruvate Carboxylase Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | – |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:100 | – |
| Immunocytochemistry (ICC/IF) | 1:50 | – |
| Immunoprecipitation (IP) | – | 1 publication |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthesized peptide derived from internal region of human Pyruvate Carboxylase (aa 357–406) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2635554 |
Product Specific Information
This antibody detects endogenous levels of total Pyruvate Carboxylase protein.
Target Information
Pyruvate Carboxylase requires biotin and ATP to catalyze the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedral structure located exclusively in the mitochondrial matrix. It is involved in gluconeogenesis, lipogenesis, insulin secretion, and synthesis of the neurotransmitter glutamate. Mutations in this gene are associated with pyruvate carboxylase deficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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