Thermo Fisher Scientific POLB Recombinant Rabbit Monoclonal Antibody (ARC0752)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:6,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:800

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

ARC0752

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 236-335 of human DNA Polymerase beta (P06746) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:POLB,uniProtId:P06746-1,ncbiNodeId:9606,antigenRange:236-335,antigenLength:335,antigenImageFileName:MA5-35359_POLB_P06746-1_Rabbit.svg,antigenImageFileNamePDP:MA5-35359_POLB_P06746-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.22 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2849260

Product Specific Information

Immunogen sequence: MGVCQLPSKN DEKEYPHRRI DIRLIPKDQY YCGVLYFTGS DIFNKNMRAH ALEKGFTINE YTIRPLGVTG VAGEPLPVDS EKDIFDYIQW KYREPKDRSE

Target Information

DNA polymerase beta comprises an amino-terminal 8- kDa domain and a carboxy-terminal 31- kDa domain. The N-terminal ssDNA binding domain has a deoxyribose phosphodiesterase activity while the C-terminal domain has a nucleotidyltransferase activity. Mammalian DNA polymerase beta, a DNA repair polymerase, is constitutively expressed in cultured cells, but treatment of cells with the DNA-alkylating agents such as N-methyl-N`-nitro-N-nitrosoguanidine (MNNG) or methyl methanesulfonate up-regulates beta-pol level. DNA polymerase beta fills single nucleotide gaps in DNA produced by the base excision repair pathway of mammalian cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.