Thermo Fisher Scientific POLB Recombinant Rabbit Monoclonal Antibody (ARC0752)
POLB 단백질을 인식하는 재조합 토끼 단클론 항체로, Western blot, IHC, ELISA에 적합. 인간, 마우스, 랫트 반응성. HEK293 세포 발현 시스템 사용. DNA 수선 관련 연구용으로 권장.
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Thermo Fisher Scientific POLB Recombinant Rabbit Monoclonal Antibody (ARC0752)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:6,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:800 |
| ELISA | 1 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | ARC0752 |
| Immunogen | Synthetic peptide corresponding to amino acids 236–335 of human DNA Polymerase beta (P06746) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.22 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol, 0.05% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2849260 |
Product Specific Information
Immunogen sequence:
MGVCQLPSKN DEKEYPHRRI DIRLIPKDQY YCGVLYFTGS DIFNKNMRAH ALEKGFTINE YTIRPLGVTG VAGEPLPVDS EKDIFDYIQW KYREPKDRSE
Target Information
DNA polymerase beta comprises an amino-terminal 8-kDa domain and a carboxy-terminal 31-kDa domain.
The N-terminal ssDNA binding domain has deoxyribose phosphodiesterase activity, while the C-terminal domain has nucleotidyltransferase activity.
Mammalian DNA polymerase beta is a DNA repair polymerase constitutively expressed in cultured cells. Treatment with DNA-alkylating agents such as N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) or methyl methanesulfonate up-regulates beta-pol levels.
DNA polymerase beta fills single nucleotide gaps in DNA produced by the base excision repair pathway of mammalian cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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