Thermo Fisher Scientific REST Recombinant Rabbit Monoclonal Antibody (ARC0755)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:6,000

-

ELISA (ELISA)

1 µg/mL

-

Immunoprecipitation (IP)

0.5 µg-4 µg antibody for 200 µg-400 µg extracts of

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

ARC0755

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 100-200 of human REST/NRSF (Q13127) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:REST,uniProtId:Q13127-1,ncbiNodeId:9606,antigenRange:100-200,antigenLength:1097,antigenImageFileName:MA5-35361_REST_Q13127-1_Rabbit.svg,antigenImageFileNamePDP:MA5-35361_REST_Q13127-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.27 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2849262

Product Specific Information

Immunogen sequence: KGEPHGLENM ELRSLELSVV EPQPVFEASG APDIYSSNKD LPPETPGAED KGKSSKTKPF RCKPCQYEAE SEEQFVHHIR VHSAKKFFVE ESAEKQAKAR E

Target Information

Transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Restricts the expression of neuronal genes by associating with two distinct corepressors, mSin3 and CoREST, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.