Thermo Fisher Scientific REST Recombinant Rabbit Monoclonal Antibody (ARC0755)
REST 단백질을 인식하는 재조합 토끼 단일클론 항체로, 인간, 생쥐, 랫드 시료에 반응합니다. Western blot, ELISA, IP에 적합하며 고순도의 Affinity chromatography 정제 항체입니다. PBS/glycerol buffer에 보관되며 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution / Amount |
|---|---|
| Western Blot (WB) | 1:1,000–1:6,000 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5–4 µg antibody for 200–400 µg extracts |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | ARC0755 |
| Immunogen | Synthetic peptide corresponding to amino acids 100–200 of human REST/NRSF (UniProt ID: Q13127) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.27 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol, 0.05% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2849262 |
Product Specific Information
Immunogen sequence:
KGEPHGLENM ELRSLELSVV EPQPVFEASG APDIYSSNKD LPPETPGAED KGKSSKTKPF RCKPCQYEAE SEEQFVHHIR VHSAKKFFVE ESAEKQAKAR E
Target Information
REST (RE1-silencing transcription factor) is a transcriptional repressor that binds neuron-restrictive silencer elements (NRSE) and represses neuronal gene transcription in non-neuronal cells. It restricts neuronal gene expression by associating with corepressors such as mSin3 and CoREST, which recruit histone deacetylase complexes to REST-regulated promoters. REST mediates repression by recruiting the BHC complex at RE1/NRSE sites, functioning as a chromatin modifier through histone deacetylation and demethylation.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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