Thermo Fisher Scientific Farnesyl Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

-

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Immunocytochemistry (ICC/IF)

Assay-dependent

-

ELISA (ELISA)

Assay-dependent

-

Product Specifications

Species Reactivity

Chemical

Published species

Chemical

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Farnesyl cysteine conjugated to KLH

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Ammonium sulfate precipitation

Storage buffer

PBS

Contains

0.08% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1074362

Target Information

Isoprenylation/methylation is an important posttranslational lipid modification: the covalent addition of a 15 carbon farnesyl or a 20-carbon geranylgeranyl group to the C-terminus of a protein results in a substantial increase in hydrophobicity. All known G proteins are modified in this way, making the pathway of central interest for an understanding of signal transduction. From nematode to human, many eukaryotic proteins are reported to be isoprenylated. Known prenylated prtoteins include fungal mating factors, nuclear lamins, Ras and Ras-related GTP-binding proteins (G proteins), and the subunits of trimeric G proteins, protein kinases and at least one viral prtoein. Prenylation promotes membrane interactions of most of these proteins, which is not surprising given the hydrophobicity of the lipids involved. In addition, prenylation appears to play a major role in several protein-protein interaction.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.