Takara Stellar chemically competent cells for cloning

Overview

Stellar Competent Cells

• Transformation efficiency: >5 × 108 cfu/µg (may improve with reduced heat-shock time)
• Genotype: F-, endA1, supE44, thi-1, recA1, relA1, gyrA96, phoA, Φ80d lacZΔ M15, Δ(lacZYA-argF) U169, Δ(mrr-hsdRMS-mcrBC), ΔmcrA, λ-

Stellar Competent Cells (dam−/dcm−)

• Transformation efficiency: >1 × 106 cfu/µg (may improve with reduced heat-shock time)
• Genotype: F-, ara, Δ(lac-proAB) [Φ80d lacZΔM15], rpsL(str), thi, Δ(mrr-hsdRMS-mcrBC), ΔmcrA, dam, dcm

More Information

Applications

• Cloning, subcloning
• Methylated DNA cloning
• cDNA and genomic library construction
• Longer-length cDNA library construction

Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.