Thermo Fisher Scientific MBD1 Monoclonal Antibody (OTI2H5), TrueMAB

Applications and Tested Dilutions

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

Methyl-CpG-binding domain protein 1 (MBD1) is a member of a family of mammalian proteins that specifically bind methylated regions of DNA.
It functions as a transcriptional repressor, binding to CpG islands in methylated promoters. Binding is inhibited by the presence of 7-mG produced by DNA damage (e.g., methylmethanesulfonate).
MBD1 contributes to gene silencing by recruiting AFT7IP, which in turn recruits histone methyltransferase SETDB1. Isoforms 1 and 2 can also repress transcription from unmethylated promoters.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.