Thermo Fisher Scientific Phospho-ELK1 (Ser389) Polyclonal Antibody
Phospho-ELK1 (Ser389) 특이적 폴리클로날 항체로, Western blot, IHC, ICC/IF에 사용 가능. Human 및 Mouse 반응성. 항원 친화 크로마토그래피로 정제되었으며, 액상 형태로 제공. 연구용으로 ELK1 인산화 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–10 µg/mL |
| Immunocytochemistry (ICC/IF) | 1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to the epitope surrounding phosphorylation site serine 389 of human ELK1 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 25 mM Tris with proprietary stabilizer |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Positive Control: Human breast carcinoma
- Cellular Location: Nucleus
Target Information
ELK1 is a component of the ternary complex that binds the serum response element (SRE) and mediates gene activity in response to serum and growth factors.
It is phosphorylated by MAP kinase pathways at a cluster of serine/threonine motifs at its C-terminus.
Phosphorylation at these sites, particularly Ser383, is critical for transcriptional activation by ELK1.
ELK1 serves as a direct target of activated MAP kinase, correlating with MAP kinase activity, and is a nuclear target in the Ras-Raf-MAPK signaling cascade.
Alternatively spliced transcript variants encoding the same protein have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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