Thermo Fisher Scientific kNBC Polyclonal Antibody
Western blot, IHC, ICC, ELISA 등 다양한 응용에 적합한 Rabbit Polyclonal 항체. Human, Mouse, Rat에 반응하며, KLH-conjugated synthetic peptide를 면역원으로 사용. 고순도의 Protein A 정제 및 안정한 보관용 buffer 구성.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:400 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic peptide derived from Mouse electrogenic sodium bicarbonate cotransporter isoform 2 (amino acids 1–100) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Gene: Slc4a4
Protein Function: Encodes a sodium bicarbonate cotransporter (NBC) involved in bicarbonate secretion, absorption, and intracellular pH regulation. It functions as an electrogenic sodium/bicarbonate cotransporter with a Na⁺:HCO₃⁻ stoichiometry of 1:2 to 1:3.
Isoforms: Isoform 2 may have higher activity than isoform 1.
Clinical Relevance: Mutations in Slc4a4 are associated with proximal renal tubular acidosis and ocular abnormalities. Multiple transcript variants encoding different isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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