Thermo Fisher Scientific Phospho-NEK2 (Ser171) Polyclonal Antibody
NEK2 단백질의 Ser171 인산화 부위를 특이적으로 인식하는 폴리클로날 항체입니다. Western blot, IHC, ICC 등 다양한 응용에 적합합니다. 사람, 마우스, 랫트 시료에 반응하며, 세포 주기 조절 연구에 활용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | - |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 | - |
Product Specifications
| Category | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human NEK2 (Accession P51955), corresponding to amino acid residues around phosphorylated Ser171. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2816760 |
Product Specific Information
This antibody detects endogenous levels of NEK2 only when phosphorylated at Ser171.
Target Information
NEK2 is a serine/threonine-protein kinase involved in cell cycle regulation and essential for accurate chromosome segregation. It is primarily localized in the centrosome. During the G1 phase, NEK2 is undetectable, but its expression increases during the S phase and peaks in late G2 phase. The gene produces multiple isoforms through alternative splicing and is associated with diseases such as Retinitis Pigmentosa 67.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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