
Thermo Fisher Scientific BMI1 Recombinant Rabbit Monoclonal Antibody (BLR119H)
BMI1 단백질을 인식하는 재조합 토끼 단일클론 항체로, Western blot, IHC, ICC, Flow cytometry, IP에 적합합니다. HEK293F 발현 시스템에서 생산되며, 인간과 생쥐 시료에 반응합니다. 1 mg/mL 농도의 액상 형태로 제공되며, 4°C에서 보관합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| Flow Cytometry (Flow) | 0.5 µL/10⁶ cells |
| Immunoprecipitation (IP) | 6 µL/mg of lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293F |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | BLR119H |
| Immunogen | Peptide representing a region between residue 250 and 300 of Human polycomb complex protein BMI1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Purified |
| Storage Buffer | BBS, pH 8.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For immunocytochemistry, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE cell sections.
Target Information
Component of the Polycomb group (PcG) multiprotein PRC1 complex, which maintains the transcriptionally repressive state of many genes, including Hox genes, throughout development. The PRC1 complex acts via chromatin remodeling and histone modification, mediating monoubiquitination of histone H2A at Lys-119. Within the PRC1 complex, BMI1 is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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