Thermo Fisher Scientific FOXO4 Polyclonal Antibody
FOXO4 단백질을 인식하는 Rabbit Polyclonal 항체로 WB, IHC, ICC/IF에 적합. Human FOXO4(aa 268–505) 재조합 단편을 면역원으로 사용. 다양한 세포주에서 검증되었으며 Mouse, Rat, Bovine에 예측 반응성. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 268–505 of Human FOXO4 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.68 mg/mL |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2546403 |
Product Specific Information
- Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji
- Predicted reactivity: Mouse (85%), Rat (84%), Bovine (88%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
FOXO4 (Forkhead box O4) is encoded by the FOXO4 gene located on the X chromosome. Also known as AFX1 or MLLT7, it is a member of the O class of forkhead/winged helix family of transcription factors along with FOXO1, FOXO3, and FOXO6.
FOXO4 is abundantly expressed in skeletal muscle and adipose tissue. It binds to the Insulin Responsive Element (IRE) and activates downstream targets of Insulin/IGF1 signaling such as IGFBP1. Activation of Akt leads to phosphorylation of FOXO4, promoting its binding to 14-3-3 proteins and export from the nucleus.
Safety Information
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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