Thermo Fisher Scientific CD14 Monoclonal Antibody (MEM-15), NovaFluor Red 710, eBioscience
인간 CD14 단백질을 인식하는 MEM-15 단클론 항체로, NovaFluor Red 710 형광체가 결합된 형광세포분석용 시약입니다. 전처리된 상태로 사용이 간편하며, 4 µL/test로 최적화되어 있습니다. NovaFluor 염료 특성상 DNA 결합 viability dye와는 비호환입니다.
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Thermo Fisher Scientific CD14 Monoclonal Antibody (MEM-15), NovaFluor Red 710, eBioscience
Applications and Tested Dilution
- Application: Flow Cytometry (Flow)
- Tested Dilution: 4 µL (0.6 µg)/test
Product Specifications
| 항목 | 내용 |
|---|---|
| Host/Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MEM-15 |
| Conjugate | NovaFluor™ Red 710 |
| Excitation/Emission Max | 637 / 713 nm |
| Form | Liquid |
| Concentration | 4 µL/Test |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2896596 |
Additional Formats Available
- NovaFluor Blue 510
- NovaFluor Blue 530
- NovaFluor Blue 585
- NovaFluor Blue 610-30S
- NovaFluor Blue 610-70S
- NovaFluor Blue 660-40S / 120S
- NovaFluor Red 700
- NovaFluor Yellow 570 / 610 / 660 / 690 / 700 / 730
- Custom conjugation available upon request
Product Specific Information
The MEM-15 monoclonal antibody reacts with human CD14, a 53–55 kDa GPI-linked glycoprotein expressed on monocytes, macrophages, and some dendritic cells. CD14 binds LPS-LBP complexes with high affinity.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Pre-diluted and tested for flow cytometry of normal human peripheral blood cells
- Recommended Use: 4 µL (0.6 µg) per 100 µL test volume (10⁵–10⁸ cells/test)
Important Notes:
- NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
- Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assessment.
- Use CellBlox Blocking Buffer (5 µL/sample) when staining with NovaFluor conjugates, including single-color controls.
Excitation: 639 nm
Emission: 710 nm
Laser: 633–640 nm (Red)
NovaFluor conjugates use Phiton™ technology, providing engineered fluorescent signatures optimized for minimal spillover and spread.
Target Information
CD14 is a 55 kDa GPI-anchored glycoprotein expressed on monocytes, macrophages, and neutrophils. It functions as a receptor for lipopolysaccharide (LPS) and is present in both membrane-bound and soluble forms. CD14 plays roles in immune recognition, inflammation, and apoptotic cell clearance. Mutations or dysregulation are associated with infections and inflammatory diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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