Thermo Fisher Scientific GTF2A2 Polyclonal Antibody
Rabbit polyclonal antibody against human GTF2A2 for Western blot applications. Affinity-purified with >95% purity. Supplied as liquid in PBS with 50% glycerol. Suitable for research use only, not for diagnostic purposes.
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Applications
- Western Blot (WB): 1:500–1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein of human GTF2A2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2719968 |
Product Specific Information
The antibody was affinity-purified from rabbit antiserum by affinity chromatography using an epitope-specific immunogen. The purity is greater than 95% as determined by SDS-PAGE.
Target Information
Accurate transcription initiation on TATA-containing class II genes involves the ordered assembly of RNA polymerase II (POLR2A) and the general initiation factors TFIIA, TFIIB, TFIID, TFIIE, TFIIF, TFIIG/TFIIJ, and TFIIH. The first step involves recognition of the TATA element by the TATA-binding subunit (TBP) and may be regulated by TFIIA, a factor that interacts with both TBP and a TBP-associated factor (TAF) in TFIID.
TFIIA has two subunits (43 and 12 kDa) in yeast and three subunits in higher eukaryotes. In HeLa extracts, it consists of a 35-kDa alpha subunit and a 19-kDa beta subunit encoded by the N- and C-terminal regions of GTF2A1, respectively, and a 12-kDa gamma subunit encoded by GTF2A2 (DeJong et al., 1995).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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