Thermo Fisher Scientific TFF2 Polyclonal Antibody

Applications

Immunohistochemistry (Paraffin) [IHC (P)]

• Tested Dilution: 15 µg/mL

Product Specifications

Product Specific Information

Percent identity with other species by BLAST analysis:

• Human, Gorilla (100%)
• Gibbon, Marmoset, Cat, Horse (90%)
• Monkey, Mouse, Pig (80%)

For IHC(P), use heat-induced antigen retrieval in pH 6.0 citrate buffer.
After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.

Target Information

Trefoil peptides are protease-resistant molecules secreted throughout the gut that play a role in mucosal healing and protection of the gastrointestinal epithelia.
These peptides contain three intrachain disulfide bonds, forming the trefoil motif or P-domain.

SP (spasmolytic polypeptide), also designated Trefoil Factor 2 (TFF2) precursor, is a trefoil protein that functions to inhibit gastrointestinal motility and gastric acid secretion.
SP may also act as a structural component of the gastric mucus, possibly by stabilizing glycoproteins in the mucus gel through interactions with carbohydrate side chains.

A downregulation of SP expression is associated with primary gastric cancer, and a progressive loss of this protein is likely to be involved in the early stage of the multi-step gastric carcinogenesis pathway.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.