Thermo Fisher Scientific ETS1 associated protein II Polyclonal Antibody, FITC
ETS1 associated protein II에 특이적인 FITC 결합 폴리클로날 항체로, Western blot, IHC, ELISA, IP에 사용 가능. 사람, 마우스, 랫트 반응성. FITC 형광(Ex/Em 498/517 nm). -20°C 보관, 암소 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (IHC) | 1:50–1:250 |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1:50–1:250 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide within amino acid region 330–360 on human Tyrosyl-DNA phosphodiesterase protein |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5–1.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer, pH 7.4–7.8, with 30% glycerol, 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
ETS1 (c-Ets-1; v-ets erythroblastosis virus E26 oncogene homolog 1 (avian); ETS-1; EWSR2; p54) is a 441 amino acid protein belonging to the DNA-binding ETS protein family. It has an N-terminal pointed domain, central transactivation domain, and two autoinhibitory domains flanking the conserved C-terminal ETS domain. ETS1 interacts with PEBP2alphaA, p300/CBP, and mediates transactivation of Osteopontin, Presenilin-1, Stromelysin, and Collagenase. Activated by Ras/MAPK signaling, its expression is upregulated in resting T-cells. Activity is modulated by AP-1, Sp-1, c-myb, and MafB. Sp100 activates ETS1 in nuclear bodies. It interacts with EAP1/Daxx to repress MMP1 and BCL2 transactivation, and with STAT6 to modulate Socs-1 expression induced by IL-4. ETS1 also interacts with GATA3 to positively regulate Tax-1 dependent IL-5 expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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