Thermo Fisher Scientific CYLD Polyclonal Antibody
Thermo Fisher Scientific의 CYLD Polyclonal Antibody는 인간 CYLD 단백질을 인식하는 토끼 폴리클로날 항체입니다. Western blot 및 ICC/IF에 적합하며, Protein G로 정제된 액상 형태입니다. 종간 반응 예측(쥐), HeLa 세포 추출물 양성 대조로 권장됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 2–4 µg/mL | - |
| Immunocytochemistry (ICC/IF) | 5–10 µg/mL | - |
| Miscellaneous PubMed (Misc) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to residues D(32) K Q T Q K L L K V P K G S(45) of human CYLD |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2261341 |
Product Specific Information
- This antibody is predicted to react with rat based on sequence homology.
- Suggested positive control: HeLa whole cell extract.
Target Information
CYLD is a 956 amino acid cytoplasmic deubiquitinating enzyme belonging to the ubiquitin carboxy-terminal hydrolases (UCH) family.
It contains three CAP-GLY domains, a proline-rich region, a SH3 binding domain, and a catalytic domain homologous to UCH.
CYLD functions as a tumor suppressor protein influencing the JNK signaling pathway and acts as a negative regulator of TRAF2 and NF-κB signaling.
It also regulates the I-κB kinase pathway and exhibits deubiquitinating activity toward non-Lys-48-linked polyubiquitin chains.
Mutations in CYLD are associated with cylindromatosis, multiple familial trichoepithelioma, and Brooke-Spiegler syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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