Thermo Fisher Scientific Luciferase Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:5,000

-

Immunohistochemistry (IHC)

1:500-1:2,500

-

ELISA (ELISA)

1:5,000-1:25,000

-

Immunoprecipitation (IP)

1:1,000-1:5,000

-

Dot blot (DB)

1:1,000

-

Product Specifications

Species Reactivity

Luciferase

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Luciferase [Photobacterium fischerii]

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

75 mg/mL

Storage buffer

0.02M potassium phosphate/whole serum, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Luciferase [Photobacterium fischerii]. Cross reactivity against Luciferase from other tissues and species may occur but have not been specifically determined.

Target Information

Luciferase produces green light with the wavelength of 562 nm. Luciferase catalyzes the reaction: D-firefly luciferin + O2 + ATP = firefly oxyluciferin + CO2 + AMP + diphosphate + light.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.